Introduction

The ZytoFast® products are designed for fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-κ and Ig-λ light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded (FFPE) tissue sections. The signal intensity of ZytoFast® probes is increased even more when using the  ZytoFast® PLUS Implementation Kits.

Method Description – ZytoFast®

The ZytoFast® Ig-kappa/Ig-lambda Probe uses oligonucleotide probes tagged with Digoxigenin and Biotin to determine the IGK and IGL clonality at once with a Dual Color CISH-System. Digoxigenin is detected by HRP-conjugated antibodies while Biotin is detected using AP-conjugated streptavidin. The enzymatic reaction of chromogenic substrates, e.g. HRP-Green and Permanent Red, leads to the formation of strong color precipitates that can be visualized by light microscopy.

Method Description – ZytoFast® PLUS

The ZytoFast® PLUS system uses Digoxigenin-labeled probes which are detected using primary antibodies. These antibodies are detected by polymerized enzyme-conjugated secondary antibodies. The enzymatic reaction of chromogenic substrates, e.g. NBT/BCIP (  ), Permanent Red (  ) or DAB (  ), leads to the formation of strong color precipitates that can be visualized by light microscopy.