Chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH) are both molecular biology techniques used to detect and visualize specific nucleic acid sequences within cells and tissues.
CISH is commonly used in the field of pathology for the detection of mutations or genome amplifications in specific biopsy tissue samples or cancer cells. In CISH, specific DNA or RNA probes are used to bind and detect the target nucleic acid sequence within the cell.
In practice, CISH is often used to detect mutations in cancer cells, including breast and prostate cancer. This technique can also be used for the diagnosis and treatment of infectious diseases, such as hepatitis virus and human papillomavirus (HPV).
Fluorescence in situ hybridization (FISH) is a molecular biology technique used to visualize and map specific DNA or RNA sequences in cells and tissues. This technique is based on the hybridization of fluorescently labeled DNA or RNA probes to the complementary nucleic acid sequence of interest.
In FISH, a fluorescently labeled probe is designed to target a specific region of DNA or RNA within a sample. The probe is then hybridized to the target sequence, allowing it to bind and fluoresce under a microscope. By visualizing the location and intensity of the fluorescent signal, researchers can determine the location and copy number of the target sequence within the cell or tissue.
Both CISH and FISH have a wide range of applications in various fields of research and clinical practice. They are commonly used in cancer research to detect genetic abnormalities and identify therapeutic targets. They can also be used in infectious disease research to identify specific pathogens or detect genetic mutations associated with drug resistance.
Biozatix Indonesia is proud to offer a wide range of molecular biology products, including chromogenic and fluorescence in situ hybridization (CISH and FISH) products. Our CISH and FISH products are designed to detect and visualize specific nucleic acid sequences within cells and tissues, making them invaluable tools for research and clinical applications.
At Biozatix Indonesia, we understand the importance of reliable and accurate molecular biology techniques, which is why we offer only the highest quality CISH and FISH products from trusted manufacturers. Our team of experts is always on hand to provide technical support and guidance to ensure the success of your experiments.
Whether you are working in cancer research, infectious disease research, or any other field of molecular biology, our CISH and FISH products can help you detect and visualize specific nucleic acid sequences with ease and confidence. Contact us today to learn more about our products and services.